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Sentences for "codon "
Codon usage comparison Codon usage varies among organisms.
The exact causes for tRNA regulation and codon usage are not completely understood, however, expression of foreign genes in an organism is often prevented by a different codon usage of the foreign gene [ 31 ] .
Many Archaea have codon usage frequencies and tRNA compositions different from E.
coli the same codon (AUA) is a rare codon present at a frequency of only 5.
The other two major differences in codon usage between the T.
Sequencing gels were ran and processed in the Biotech Center (University of Connecticut) Codon usage The program Codon Usage Tabulated from GenBank (CUTG) at http://www.
jp/codon/ [ 43 ] was used to calculate the codon usage of individual genes and genomes.
To express the 67 kDa N-terminal transesterification domain (Top67), a stop codon at amino acid 598 was introduced into plasmid pJW312 [ 42 ] used for topoisomerase I expression by site-directed mutagenesis employing the Chameleon-Mutagenesis kit from Stratagene.
The pKK-RT66 clone was digested with HpaI restriction enzyme to generate a blunt end at codon 136.
The 18-mer duplex DNA encoding the insertion peptide (Ile-Asn-Asn-Glu-Thr-Ile) was ligated with Hpa1 digested pKK-RT66 in between codon 135 and 136.
A unique Sac I site was also introduced in pKK-RT66 template at codon 440.
The antiserum detects a protein of 55 KD (predicted size of the plasma membrane thiamine transporter) that is not detected in cells from TRMA individuals that possess a premature stop codon within the transporter gene (unpublished results).
The cornerstone of selection mapping is the testing of each observed replacement mutation at each codon to identify those particular replacement mutations that are overabundant relative to silent mutations at that codon.
For example, we would predict that the anti-CD4BS Abs will have particular trouble recognizing gp120 molecules carrying the positively selected variant of the CD4BS epitope (D›N at codon 474).
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