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Example Sentences for "collagenase "


These include motifs for transcription factors that bind to collagenase and elastase genes and motifs for the transcription factor MEP-1, which regulates transcription from the stromelysin-1 (MMP-3) and metallothionein genes [ 18, 19].

In contrast, the expression of meltri n-ß and stromelysi n- 2 was similar in all samples and expression of collagenase I or IV was undetectable in either sense or antisense-expressing aggregates (not shown).

Expression of all MMPs was undetectable except for collagenase I.

Synthesis of MMPs such as collagenase I (MMP-1), gelatinase B (MMP-9) and matrilysin (MMP-7) has been shown to be dependent on the synthesis of PG E 2 , suggesting that alterations in MMP expression may be instigated by alterations in PG synthesis [ 27, 28, 29, 30].

RAW transfectants over-expressing IDO showed reduced expression of collagenase I (MMP-1), and also bound less well to collagen-coated plates than controls.

Therefore, we tested whether cCAF affects the production or activity of the gelatinases MMP-2 or MMP-9, or the collagenase MMP-1, all of which digest collagens I and III.

However, immunoblot analysis using a polyclonal antibody to human MMP-1 (this reagent is not available for the chicken homologue of this matrix metalloproteinase) showed that cCAF treatment caused a decrease in the amount of the collagenase MMP-1 detected, but neither TIMP-1 nor TIMP-2 (two natural inhibitors of these MMPs) levels were changed, as evaluated by immunoblot analysis with a monoclonal antibody to the corresponding human proteins (Fig.

The former investigators reported that MCP-1 increases collagen accumulation not by decreasing the levels of the collagenase MMP-1, but by stimulating the production of TGFß1, a well-known stimulator of collagen expression and expression of TIMPs, the natural inhibitors of MMPs.

Bone-forming cells were isolated from mouse neonate calvariae by sequential collagenase and protease digestions.

After gross mechanical isolation, individual oocytes were defolliculated by incubation with collagenase 1A (1 mg/mL) in Ca 2+-free OR2 medium (82.

Further, tissues were cut into smaller pieces and incubated at 37°C in Isocove's medium supplemented with 10% fetal calf serum and penicillin/streptomycin containing 400 U/ml Collagenase D (Boerrhinghem & Mannheim GmbH, Mannheim, Germany) and 25 U/ml DNAse (Worthington Biochemical, Lakewood, NJ) for 2-3 hours.

The epidermis was then removed with forceps and the dermal pieces were incubated with Collagenase D (3 mg/ml in collagenase buffer (see below)) for 15-30 min at 37°C.

25 mM EDTA) for 10 min at 37°C, and then with 400 µl collagenase D (5 mg/ml, Boehringer) in collagenase buffer (20 mM Hepes pH 7, 0.

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