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Sentences for "collagenous "
However, as TGFß1 regulates the expression and deposition of collagenous extracellular matrix (ECM) [ 12 13 14 ] it is expected that keloids develop due to aberrant responses to this cytokine.
To determine the effect of CPT on newly synthesized collagenous proteins, we used an established method of metabolic labeling with [ 3H]proline followed by SDS-PAGE electrophoresis [ 22].
The intensity of bands of collagenous protein was quantitated using NIH-Image (Densitometry Software, version 1.
The effect of CPT on newly synthesized collagenous proteins secreted into the media was examined in five pairs of SSc and healthy control fibroblasts in nine independent experiments.
CPT at concentrations from 10 -8to 10 -6mol/l significantly decreased secretion of collagenous protein, by up to 50% in both cell types (Fig.
Secreted collagenous proteins were inhibited by 29% ± 0.
Taken together, the experiments presented in this part of the study suggest that CPT is a relatively specific inhibitor of collagenous protein production in dermal fibroblasts.
For determination of newly synthesized collagenous proteins, fibroblasts were plated in 12-well plates and grown to visual confluency.
The intensity of collagenous protein bands was quantitated using NIH-Image.
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