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Sentences for "colorimetric "
The production of Collagen type II was assessed by immunohistochemistry using a monoclonal antibody against mouse type II Collagen (Chemicon), and secondary antibody and colorimetric reaction kit (Histostain Plus, Zymed).
For colorimetric detection, hybridization with the targets was revealed by reacting the tissue sections with anti-DIG-alkaline phosphatase conjugate, followed by reaction with nitroblue tetrazolium chloride (NBT), 5-bromo-4-chloro-3-indolyl phosphate (BCIP), and levamisole as previously described [ 39].
Experimental protocol to determine the viability of domoic acid-treated rat neonatal microglia or cerebellar granule neurons A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.
Membrane protein concentrations were determined by a colorimetric assay according to manufacturer's protocol (BioRad, Hercules, CA) and 20 µg of total membrane proteins resolved by 7.
LDH release was quantified using a commercially available colorimetric kit (Sigma, St.
Viability measurements Neuronal viability was assessed using the colorimetric MTT assay as described previously [ 14 ] .
AP activity in the supernatants was estimated using a p -nitro-phenol colorimetric assay.
The levels of sulfated GAGs in lavage fluids were determined using a colorimetric dye-binding assay using 1,9-dimethylmethylene blue [ 23 ] .
Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
This overall depiction is called a colorimetric comparison array (CCA).
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