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Sentences for "complementation "
The complementation assay shown in Fig.
Further, mutations that have little effect on APE activity in vitro prevent complementation of DNA repair deficient E.
We report here the complete mutant screen identifying 121 individual mutants that subdivide into at least 11 complementation groups.
Based on pairwise crosses of mutants with opposite mating type, only one-third (37/121) of the mutants were initially catalogued into 8 different complementation groups (Figure 1B).
The remaining two-thirds (84/121) of the mutants could not be assigned to a complementation group.
Subsequent backcrossing and isolation of opposite mating type strains for the some of the mutants allowed definition of an additional complementation group.
Nup localization analysis in npastrains To investigate the effects on NPCs more directly, we first targeted members of the largest independent complementation groups for analysis.
Interestingly, mutant alleles in the two largest complementation groups showed a similar pattern of GFP-Nup mislocalization.
Other members of the npa1 and npa2 complementation groups showed similar phenotypes (data not shown).
Identification of mutants To identify the mutated genes, a yeast genomic library was screened for complementation of the recessive ts phenotypes of representative mutants.
Based on the GFP-Nup localization and genetic analysis, a range of mutant phenotypes and complementation groups were catalogued.
Several of the complementation groups contained multiple independent alleles ( npa1-9 ), and within a single complementation group the strengths of the defects were varied.
However, there was only one allele for the majority of the complementation groups, representing potentially npa10-87.
Here we focused on characterizing the largest complementation groups.
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