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Sentences for "conceptuses "
Results indicate expression of RAR?
and RBP increase during transition to conceptuses of filamentous morphology.
Due to the limited time frame when conceptuses are in tubular transitional development (2-3 hrs) and difficulty in determining when tubular conceptuses are in the uterus following mating, one uterine horn was removed on day 11.
Conceptuses were flushed into a sterile petri dish from the uterine horn and evaluated to determine an appropriate time-delayed removal of the second horn corresponding to the predicted time conceptuses would be in a tubular morphology as described by Geisert et al.
Morphology of conceptuses collected following flushing from the uterine horns was recorded and conceptuses of identical morphologies were transferred to cryogenic vials, snap-frozen in liquid nitrogen, and stored at -80°C until extracted for RNA.
RNA Isolation Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] .
The second comparison was made between tubular (15-40 mm) and filamentous (>150 mm) morphologies encompassing gene expression changes during the transitional completion when the conceptuses have began to cover the surface area of the uterine horns.
Individual and small pools of conceptuses were evaluated at the four morphologically distinct stages, spherical (n = 8), ovoid (n = 2), tubular (n = 5) and early filamentous (n = 6).
Numerous transcripts were up-regulated in conceptuses of filamentous morphology including, pro-interleukin-1ß, interleukin-1ß (IL-1ß), s-adenosylhomocysteine hydrolase (SAHH), heat shock cognate 70 kDa protein (HSC70), metallopanstimulin-1 (MPS-1) and elfin.
01) in filamentous conceptuses compared to all other morphologies evaluated (Table 4).
There was an approximately 7-fold increase of SAHH gene expression in filamentous conceptuses compared to spherical conceptuses (Figure 1).
004) in tubular conceptuses compared to spherical while filamentous conceptus expression was greater (P < 0.
An autocrine effect of conceptus estrogen synthesis has been suggested as filamentous conceptuses have greater gene expression for estrogen receptor ß compared to its spherical and tubular counterparts [ 9 ] .
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