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Sentences for "condensate "
Exposure of epithelial cells to cigarette smoke condensate Cultures of A549 cells or NHBEs were grown to 50-75% confluence, then incubated with serum-deficient media for 4 hours before exposure to CSC.
Results Effects of cigarette smoke condensate on cell viability and apoptosis To determine the effects of CSC on cell viability, A549 cells and NHBEs were exposed to increasing concentrations of CSC for 30 min then examined using the trypan blue dye-exclusion test (Fig.
Changes in expression of specific cytokines and chemokines after exposure to cigarette smoke condensate To characterize the response of epithelial cells to CSC we determined the changes in message levels for a group of cytokines, chemokines and cell-adhesion molecules.
Involvement of the MAPK pathway in response to cigarette smoke condensate To examine if CSC-upregulation of cytokine production involves activation of MAPKs we examined CSC-treated NHBEs for specific phosphorylated species.
Cigarette smoke condensate treatment induces rapid activation of the NF?
B pathway To determine whether upregulation of cytokine production by CSC involved activation of NF?
B, we examined CSC-treated epithelial cells for phosphorylation of the inhibitor of NF?
B (I?
B).
Effect of cigarette smoke condensate on transcriptional activation of luciferase reporter construct CSC appears to activate NF?
B, which should result in transcriptional activation of NF?
B-inducible genes.
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