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Sentences for "confocal "
The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (made for Affymetrix by Hewlett-Packard).
Following an additional 2 day of culture, the coverslips containing the attached BPAECs were fixed, stained with rhodamine-phalloidin, and mounted onto slides for analysis by confocal microscopy.
Since the cells were evenly distributed throughout the area of the coverslip exposed to flow, representative pictures were taken of the confocal microscopy fields located directly in the center of the coverslip, and the number of cells were counted.
Representative confocal microscopy fields for both control and resveratrol-treated cells were counted for elongated and non-elongated cells.
Twenty-four h after seeding, the coverslips were treated with 0,10, 25, 50, and 100 µM resveratrol and maintained for 2 days at 37°C until they were fixed and stained for confocal microscopy.
Confocal microscopy Prepared slides containing adhered BPAEC were examined using a BioRad confocal microscope.
Fixing and staining of cells for confocal microscopy was as described above.
Our experiments were focused on the analysis of microextensions and their cytoskeletal core by SEM and confocal laser scanning microscopy by searching for a new fixation protocol.
Cells were washed three times in PBS for 5 min and rinsed with water at room temperature, mounted in PermaFluor (Shandon, Pittsburgh, PA) and observed under a laser scanning confocal microscope (MRC-1024, Bio-Rad Laboratories, Richmond, CA).
Immunocytochemistry and confocal microscopy Immunocytochemistry was carried out as described previously [ 40 ] .
Confocal Microscopy was carried out on a Leica TCS 4D Confocal Laser Scanning Microscope at the Schepens Eye Research Institute (Boston MA).
Samples were analyzed with a confocal microscope and cells were identified as apoptotic only when they were double labeled.
Double confocal immunofluoresence of mouse tissue (embryonic day 15, El 5) demonstrated that MAP1b immunoreactive axons of the vomeronasal nerve (VNN), the highway for GnRH neurons in the mouse [ 11 13 ] express PN-1 protein (Figure 4) but not trypsin (data not shown).
All fluorescent images were acquired with a Zeiss confocal microscope using LSM 410.
Microscopy Confocal images were obtained using a BioRad confocal microscope at the Keck Center for Biological Imaging at the University of Virginia.
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