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Afterwards long elliptical sporules of the second generation are produced on short pedicels by the conjugated fusiform bodies of the first generation.

The zone from whence the processes issue, and also the processes themselves, have their walls blackened deeply, while the walls of the conjugated cells, which continue to clothe the zygospore during the whole of its development, are bluish-black.

From the first these two processes are applied firmly one to the other by their extremities; they increase in size, become clavate, and constitute together a fusiform body placed across the two conjugated filaments.

The two conjugated cells of the fusiform body are generally unequal; the one is a cylinder as long as it is broad, the other is disciform, and its length is only equal to half its breadth.

It is rare that there is not a considerable difference of size between the two conjugated cells and the suspenders.

If the plastic matters contained in the conjugated cells influence one another reciprocally, no notable modification in their appearance results at first.

Subsequent incubation with anti-?

3 mAb directly conjugated to Alexa-488 revealed that ?

3ß1 was highly clustered (Fig.

After preincubation with protein A-sepharose 4CL, the cell extract was incubated with specific mAb directly conjugated to sepharose 4B for 60 min, washed 3 times with the appropriate lysis buffer and eluted with Laemmli sample buffer.

Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.

These bile acids are conjugated to either taurine or glycine before secretion into bile [ 4 ] , which is subsequently released into the small intestine.

The apical sodium/bile acid co-transporter (asbt) mediates the active uptake of conjugated bile acids in the ileum [ 5 6 ] .

Of these, the sodium/bile acid co-transporting polypeptide (ntcp) is considered to be quantitatively the most important transporter of conjugated bile acids in these cells [ 7 8 ] .

Thus, different classes of conjugated bile acids stimulate distinctive responses in McNtcp cells.

24 cells to conjugated bile acids is due to the existence of a specific mechanism that is capable of recognizing different classes of conjugated bile acids.

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