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24 cells increased by 20 to 31% within 2 h of incubating the cells in medium containing 100 µM of glycine conjugates of cholic acid, chenodeoxycholic acid, and deoxycholic acid (GCA, GCDCA, GDCA, respectively) (Figure 4A).

recently demonstrated that partially purified CSN promotes the cleavage of Pcu1p-Ned8p conjugates in vitro [ 10], but it remained unclear whether the enzymatic activity is contained within one of the CSN subunits or a tightly associated peptidase.

All peptidases known to cleave ubiquitin- or SUMO-protein conjugates are cysteine-based proteases (reviewed in ref.

The sizes of adenovirus-microbead conjugates are smaller than those of target cells.

Thus, the entire adenovirus-microbead conjugates can be taken up by cells.

This may be one possible explanation of why infectivity enhancement was seen with adenovirus-microbead conjugates and was not with adenoviral vectors immobilized on streptavidin-coated wells, except for one cell line (C6).

In order to observe the direct-labeling of the SP conjugates to the SPR, CHO cells expressing the rat SPR (rSPR), were incubated with the SP analogs (100 nM) for 2 hrs at 4°C, to prevent internalization of the receptor, rinsed and fixed.

SP receptor binding An important determinant of the usefulness of the SP conjugates is their ability to bind to and activate the SPR.

Statistical analysis also demonstrated that the competition binding curves generated by the SP conjugates were significantly different than the unlabeled SP binding curve (p < 0.

The biological activity of the SP conjugates was tested by examining their ability to elicit Ca ++elevations.

Of the conjugates that competed with [ 125I] SP, fluorescein-SP had the lowest binding affinity as compared to unlabeled SP.

Binding analysis showed that the above conjugates compete with [ 125I] SP at the rSPR.

Statistical analysis showed that there was not a significant difference between the fluorescent conjugates and unlabeled SP when 1 µM of SP or its derivatives was present with 50 pM [ 125I] SP.

In the first control experiment, untransfected CHO cells were utilized to determine if any of the SP conjugates were eliciting Ca ++responses through a pathway independent of rSPR activation.

The liver first conjugates them, then the kidney excretes 90% to 95% of the metabolites.

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