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Sentences for "constitutively "
In this report we demonstrate for the first time that CD98 constitutively and specifically associates with intact ß1 integrin heterodimers.
Because not all ß1 integrins associated constitutively with CD98 (in the absence of manganese), the ?
chain must help regulate specificity of CD98 association.
In addition, we have constitutively overexpressed IDO in adherent and non-adherent cell lines in vitro.
Inhibition of endogenous IDO expression disrupts P19 cell adhesion To examine the role of IDO expression in P19 cells, we transfected P19 cells with a DNA construct which contained a 740 bp fragment of murine IDO cDNA in the antisense orientation, under the control of the constitutively active CMV promoter in the pcDNA-3 mammalian expression vector.
2C, 2D), where both cullins seem constitutively neddylated, argues against a critical role for dynamic cycles of neddylation and deneddylation in cullin function in fission yeast.
10) have various expression levels of doubly spliced viral mRNAs, including Tat, Rev, Nef, Vpr, and other accessory proteins, we decided to perform the microarray in a system where Tat was constitutively expressed, asking whether Tat by itself, or in the absence of other accessory proteins, could still down-regulate host cellular genes.
Virus infectivity can be enhanced by treatment of cells with MAPK stimulators, such as serum and phorbol myristate acetate, as well as by coexpression of constitutively activated Ras, Raf, or MEK in the absence of extracellular stimulation [ 61 ] .
Interestingly, the anti-phospho-ERK immunoblots revealed that ERKs were constitutively activated in untreated, quiesced MDCK cells and activation was not enhanced further by ATP or IONO (Fig.
1C), which is consistent with the observation that ERKs are constitutively activated.
ERK was found to be constitutively phosphorylated in our study using MDCK cells from ATCC, in contrast to what has been reported previously for MDCK-D 1 , a subclone of MDCK selected for adrenergic receptor expression [ 39 40 ] .
In MDCK-D 1 cells, without constitutively active ERKs, AA release is delayed after [Ca 2+] i mobilization, and is temporally correlated with ERK activation [ 40 ] , whereas in MDCK cells with constitutively active ERKs, AA release is rapid, with significant AA release measured 30 s after [Ca 2+] i increase [ 15 ] .
Its transcript levels are significantly upregulated in response to pheromone, but are unchanged in tec1?
strains [ 6 ] , that study notes, however, that in dig1?
dig2?
cells, fus1 is constitutively activated, and both mating and invasive growth are observed.
Furthermore, experimental evidence suggests that when a signaling network is activated, positive feedback mechanisms upregulate the expression of genes that encode pathway proteins [ 26 ] , implying that this rationale is also applicable to "surveillance" pathways, whose protein components may need to be constitutively present in small quantities, but whose concentration increases with activation.
In vivo footprinting analysis shows that SRF is constitutively bound to the SRE in both quiescent and growth factor stimulated cells [ 6].
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